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Individual promoter and intron p53‐binding motifs from the rat Cyclin G1 promoter region support transcriptional activation by p53 but do not show co‐operative activation
Author(s) -
Yardley Gina,
Zauberman Arie,
Oren Moshe,
Jackson Paul
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00650-4
Subject(s) - transfection , promoter , transcription (linguistics) , intron , microbiology and biotechnology , plasmid , biology , gene , reporter gene , transcription factor , gene expression , genetics , linguistics , philosophy
The rat Cyclin G1 gene promoter contains one p53‐binding motif upstream of the transcription start site, and a second motif downstream in the first intron. We have investigated the possibility that these motifs co‐operate to permit high level promoter activation by p53. Although individual motifs supported p53‐dependent, orientation‐independent transcriptional activation, using reporter plasmids containing both motifs, we found no evidence for co‐operative promoter activation either after co‐transfection with human p53 expression plasmids, or after exposure of transfected cells to cisplatin and UV‐radiation.