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Molecular cloning of a novel GTP‐binding protein induced in fish cells by rhabdovirus infection
Author(s) -
Lee Eun-Hee,
Kim Hyun-Ju,
Park Jeong-Jae,
Choi Jeong-Yun,
Cho Wha-Ja,
Cha Seung-Ju,
Moon Chang-Hoon,
Park Jeong-Min,
Yoon Won-Joon,
Lee Byung-Ju,
Lee Dong-Hun,
Kang Ho-Sung,
Yoo Mi-Ae,
Kim Han-Do,
Park Jeong-Woo
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00641-3
Subject(s) - cloning (programming) , fish <actinopterygii> , gtp' , biology , chemistry , virology , microbiology and biotechnology , biochemistry , fishery , computer science , enzyme , programming language
We have cloned and sequenced a cDNA encoding GTP‐binding protein from a fish cell, CHSE‐214. The clone was 1493 bp long and contained an open reading frame encoding 364 amino acids. It has the five sequence motifs G1–G5 that are conserved in all GTP‐binding proteins. Its amino acid sequences are strikingly different from those of the well‐characterized G‐proteins. However, sequences closely related to this protein are found in various kinds of species including human, Arabidopsis , Drosophila and archaebacteria, suggesting a novel subfamily within the superfamily of the GTP‐binding proteins. Northern analysis indicates that this gene is constitutively expressed at a low level in normal cells but is induced by fish rhabdovirus infection at about 24 h post infection and disappears thereafter. Based on these observations, we propose that this protein represents an evolutionarily conserved novel subfamily of GTP‐binding proteins which may play an important role in fish rhabdovirus infection.