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Modification of tissue factor by peroxynitrite influences its procoagulant activity
Author(s) -
Adam J.M.,
Ettelaie C.,
Naseem K.M.,
James N.J.,
Bradley N.J.,
Bruckdorfer K.R.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00627-9
Subject(s) - peroxynitrite , chemistry , tissue factor , nitric oxide , superoxide , tyrosine , biochemistry , peroxynitrous acid , nitrotyrosine , coagulation , reactive nitrogen species , extracellular , amino acid , reactive oxygen species , nitric oxide synthase , enzyme , medicine , organic chemistry
Peroxynitrite, a reactive oxidising species resulting from a reaction between nitric oxide and the superoxide anion, modifies proteins by nitration of certain amino acids such as tyrosine. Tissue factor (TF), a transmembrane protein, is expressed on cells under inflammatory conditions and initiates the coagulation cascade. The extracellular domain of TF is rich in tyrosine. Exposure of recombinant TF and cellular TF to peroxynitrite was associated with a reduction in procoagulant activity. This was accompanied by an elevated level of nitrotyrosine residues. Peroxynitrite may have a protective role by attenuation of the thrombotic properties of TF.