Premium
Growth inhibition by overexpression of human DEAD box protein rck/p54 in cells of a guinea pig cell line
Author(s) -
Akao Yukihiro,
Mizoguchi Hiromi,
Ohishi Nobuko,
Yagi Kunio
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00605-x
Subject(s) - dead box , transformation (genetics) , cell culture , western blot , transfection , microbiology and biotechnology , biology , rna helicase a , complementary dna , cell growth , guinea pig , cell , helicase , rna , gene , biochemistry , genetics
We transfected cells of a guinea pig cell line with RCK cDNA inserted in a pIRES1neo expression vector. The overexpression of rck/p54 was confirmed by Western blot and RT‐PCR analysis. In two clones expressing rck/p54, the cell growth was highly inhibited; and their anchorage‐independent growth, which is an important character of malignant transformation, was not found. These findings are the first evidence that the overexpression of a DEAD box protein/RNA helicase could inhibit substantially cell growth at the translational level.