Premium
31 P NMR study of the interactions between oligodeoxynucleotides containing (6‐4) photoproduct and Fab fragments of monoclonal antibodies specific for (6‐4) photoproduct
Author(s) -
Torizawa Takuya,
Kato Koichi,
Kimura Yoshiko,
Asada Takako,
Kobayashi Hiroyuki,
Komatsu Yasuo,
Morioka Hiroshi,
Nikaido Osamu,
Ohtsuka Eiko,
Shimada Ichio
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00582-1
Subject(s) - adduct , chemistry , oligonucleotide , monoclonal antibody , epitope , stereochemistry , antibody , nuclear magnetic resonance spectroscopy , microbiology and biotechnology , dna , biochemistry , biology , organic chemistry , genetics
A 31 P nuclear magnetic resonance (NMR) study of the interactions between oligonucleotides containing the (6‐4) photoproduct and the Fab fragments of monoclonal antibodies (64M3 and 64M5) recognizing the (6‐4) photoproduct is reported. The 31 P chemical shift data indicate that backbone conformation of (6‐4) adduct is affected by the presence of flanking oligodeoxynucleotides, and (6‐4) adducts with different backbone conformations are accommodated in the antigen binding sites of these antibodies. It was also revealed that epitopes for these antibodies consist of not only the (6‐4) adduct but the flanking di‐ or tri‐deoxynucleotides on both the 5′ and 3′ sides as well.