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Retinoic acid regulates selectively the expression of immediate early response genes in PC12 cells
Author(s) -
Cosgaya José Miguel,
Pérez-Juste German,
Aranda Ana
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00577-8
Subject(s) - cycloheximide , retinoic acid , nerve growth factor , gene expression , messenger rna , biology , endocrinology , immediate early gene , medicine , microbiology and biotechnology , gene , chemistry , protein biosynthesis , biochemistry , receptor
Nerve growth factor (NGF) induces neuronal differentiation and growth arrest in PC12 cells. One of the initial effects of NGF in these cells is the induction of the expression of immediate early genes (IEGs). In this study we have analyzed the influence of retinoic acid (RA), which exerts important effects on PC12 cell proliferation and function, on the expression of IEGs. Incubation with RA did not alter NGFI‐A mRNA levels, but significantly reduced the NGFI‐B and c‐ fos response to NGF and serum. The response to NGF was maximal in the presence of cycloheximide, and RA also reduced the superinduction of NGFI‐B and c‐ fos mRNA levels. Sequences contained within the 5′ flanking region of the c‐ fos gene confer responsiveness to NGF and mediate the inhibitory effect of RA. The differential regulation by RA suggests that NGF induces expression of the three IEGs by different mechanisms.