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A novel importin α from rice, a component involved in the process of nuclear protein transport 1
Author(s) -
Iwasaki Toshisuke,
Matsuki Rikyu,
Shoji Kazuhiro,
Sanmiya Kazutsuka,
Miyao Mitsue,
Yamamoto Naoki
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00540-7
Subject(s) - importin , nuclear transport , nuclear localization sequence , nls , fusion protein , nuclear protein , biology , complementary dna , cytoplasm , microbiology and biotechnology , biochemistry , gene , cell nucleus , chemistry , transcription factor , recombinant dna
In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin α. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin α. Although the encoded protein had only 23–27% amino acid identity to the importin αs from various organisms including plants, the fusion protein with glutathione S‐transferase showed a specific binding activity to the NLS of SV40 T‐antigen. These results suggest that the rice #61L protein is a novel importin α in plants.