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Human/mouse interleukin‐1 receptor/receptor accessory protein interactions in IL‐1β‐induced NFκB activation
Author(s) -
Layé Sophie,
Lundkvist Johan,
Bartfai Tamas
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00537-7
Subject(s) - transfection , heterologous , hek 293 cells , microbiology and biotechnology , receptor , chemistry , biology , gene , biochemistry
We examined whether functional heterologous complexes between human IL‐1RI (hIL‐1RI) and murine IL‐1R accessory protein (mIL‐1RAcP) can be formed, utilizing human fibroblast HEK 293 cells and murine fibroblast C127 cells, non‐transfected or stably transfected with hIL‐1RI (C127‐hIL‐1RI), respectively. In non‐transfected C127 cells, IL‐1β signalled through the mIL‐1RI‐mIL‐1RAcP complex and activated NFκB p50/p65 heterodimers. In C127‐hIL‐1RI cells, IL‐1β signalled through the hIL‐1RI and activated both p65/p65 and p50/p65 NFκB complexes, where only the activation of NFκB p65/p65 was dependent on mIL‐1RAcP. Thus, clearly both homologous and heterologous IL‐1RI‐IL‐1RAcP interactions support NFκB translocation, but with differences in signalling pattern.