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Alternative promoter usage and tissue specific expression of the mouse somatostatin receptor 2 gene
Author(s) -
Kraus Jürgen,
Wöltje Michael,
Schönwetter Nadja,
Höllt Volker
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00520-1
Subject(s) - somatostatin receptor , somatostatin receptor 3 , somatostatin receptor 1 , somatostatin receptor 2 , gene expression , somatostatin , gene , biology , microbiology and biotechnology , chemistry , genetics , endocrinology
We have cloned the 5′ upstream regulatory region of the mouse somatostatin receptor 2 gene. Its genomic organization is novel among all somatostatin receptor genes. It contains two previously unrecognized exons, separated by introns larger than 25 kb, and three tissue and cell specific alternative promoters. The first promoter in front of exon 1 is active only in AtT‐20 tumor cells. The second promoter, located 5′ to exon 2, is used in brain, pituitary, adrenals, pancreas, NG 108‐15 and AtT‐20 cells. Furthermore, it contains putative DNA elements for regulation by glucocorticoids, estradiol and cAMP. A third promoter, located in exon 3, is additionally used in lung, kidney and spleen.