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The cloning and developmental regulation of murine diacylglycerol kinase ζ
Author(s) -
Ding Li,
McIntyre Thomas M,
Zimmerman Guy A,
Prescott Stephen M
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00490-6
Subject(s) - diacylglycerol kinase , biology , in situ hybridization , microbiology and biotechnology , kinase , phosphatidic acid , complementary dna , embryo , protein kinase c , messenger rna , cloning (programming) , embryonic stem cell , biochemistry , gene , phospholipid , computer science , programming language , membrane
Diacylglycerol kinases (DGKs) regulate the key signaling intermediates diacylglycerol (DAG) and phosphatidic acid (PA). We isolated cDNA clones of mouse diacylglycerol kinase ζ (mDGKζ) and found that it shares 88% identity at the nucleic acid level and 95.5% identity at the amino acid level with human DGKζ (hDGKζ). Murine DGKζ protein rose gradually during embryonic development, and was abundant in newborn and adult brains. By RNA whole‐mount in situ hybridization, mDGKζ was shown to be expressed in spinal ganglia and limb buds at low level in E11.5 embryos and at higher level in E12.5 embryos. In E13.5 embryos, DGKζ mRNA was highly expressed in vibrissa follicles, in spinal ganglia, and in the interdigital regions of the developing limbs. Northern blotting showed that DGKζ expression was limited to specific anatomical regions of the brain. Thus, the expression of DGKζ is regulated temporally and spatially during mammalian development and correlates with the development of sensory neurons and regions undergoing apoptosis.