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Metabolism of 8‐iso‐prostaglandin F 2α
Author(s) -
Basu S
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00481-5
Subject(s) - chemistry , urine , metabolite , chromatography , tritium , metabolism , urinary system , prostaglandin , prostaglandin f , endocrinology , biochemistry , medicine , physics , nuclear physics
Tritium labelled ( 1.1 MBq/17.7 μg/kg) and unlabelled 8‐iso‐PGF 2α (43 μg/kg) were administered intravenously to female rabbits and frequent blood and continuous urinary samples were collected up to 4 h. The total radioactivity was lost rapidly from the circulation. About 80% of the total radioactivity was found in urine within 4 h. The plasma half‐life of 8‐iso‐PGF 2α is found to be 1 min at the distribution phase. The terminal elimination phase half‐life was about 4 min. At 1.5 min after administration 64%, 19% and 13% of the plasma radioactivity represented 8‐iso‐PGF 2α , 15‐keto‐8‐iso‐PGF 2α and β‐oxidised products, respectively. The values for 20‐min plasma were 5%, 2% and 88%. The radiochromatograms from 10 min–4 h urinary samples were dominated by more polar β‐oxidised products. α‐Tetranor‐15‐keto‐13,14‐dihydro‐8‐iso‐PGF 2α was identified as a major urinary metabolite.Thus, 8‐iso‐PGF 2α metabolises in the rabbit mainly to several degraded polar metabolites through dehydrogenation at C‐15, reduction of Δ 13 ‐double bond and β‐oxidation, and excretes efficiently into the urine.