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Staurosporine‐sensitive protein phosphorylation is required for postreplication DNA repair in human cells
Author(s) -
Svetlova M.P,
Solovjeva L.V,
Nikiforov A.A,
Chagin V.A,
Lehmann A.R,
Tomilin N.V
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00477-3
Subject(s) - proliferating cell nuclear antigen , nucleotide excision repair , biology , postreplication repair , staurosporine , dna repair , microbiology and biotechnology , dna replication , chromatin , dna damage , dna , phosphorylation , protein kinase a , biochemistry
DNA repair is an important factor of stability of pro‐ and eukaryotic genomes which plays a central role in mutagenesis and carcinogenesis. Genetic control of nucleotide excision repair (NER) in mammalian cells is well studied, but little is known about molecular mechanisms of postreplication repair (PRR) which allows bypass of base lesions in template strands after DNA replication. In Saccharomyces cerevisiae PRR is controlled by the RAD6/RAD18 pathway which involves POL30 gene encoding proliferating cell nuclear antigen (PCNA), and in human cells PCNA is known to be closely associated with the newly replicated chromatin where PRR probably takes place. In UV‐irradiated human cells distinct PCNA foci may be detected in some cells which accumulate phosphorylated breast cancer susceptibility protein BRCA1 and another protein BARD1. Human PCNA is also known to be phosphorylated after UV‐irradiation. In this study we found that the known inhibitor of protein kinases staurosporine supresses PRR in NER‐deficient cells which is consistent with the view that BRCA1 and PCNA are required for PRR. We also have shown that the distinct PCNA foci in UV‐irradiated NER‐deficient cells are actually associated with the newly replicated chromatin. Since RAD18 protein is not essential for normal DNA replication and directly controls PRR in yeast, we analysed whether this protein as well as its human homologs (HR18A and HR18B) have common domains with BRCA1 and BARD1. It is found that HR18A has a subregion of homology to BARD1 and HR18A‐to BRCA1. Taken together the results indicate that BRCA1 and BARD1 may be involved in PRR in human cells.