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P 1 ,P 4 ‐diadenosine 5′ tetraphosphate induces nitric oxide release from bovine aortic endothelial cells
Author(s) -
Hilderman Richard H,
Christensen Eric F
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00454-2
Subject(s) - nitric oxide , egta , chemistry , vasodilation , arginine , nitric oxide synthase , endothelial stem cell , omega n methylarginine , endothelium derived relaxing factor , endothelium , biochemistry , biophysics , medicine , endocrinology , calcium , in vitro , biology , amino acid , organic chemistry
Since the infusion of P 1 ,P 4 ‐diadenosine 5′ tetraphosphate (Ap4A) into animal models induces vasodilation [1, 2], the present study was performed to determine whether Ap4A induces the release of nitric oxide (NO) from endothelial cells. Ap4A induced NO release was 4.2‐fold greater than the amount of NO released under basal condition. Ap4A induced NO release was inhibited by N G ‐nitro‐ l ‐arginine (L‐NNA) and this inhibition was reversed by L‐Arg. In addition, EGTA inhibits Ap4A induced NO release. These data are consistent with Ap4A inducing the release of NO from endothelial cells through the activation of endothelial nitric oxide synthase.