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Crystal structure of domain E of Thermus flavus 5S rRNA: a helical RNA structure including a hairpin loop
Author(s) -
Perbandt Markus,
Nolte Alexis,
Lorenz Siegfried,
Bald Rolf,
Betzel Christian,
Erdmann Volker A
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00436-0
Subject(s) - thermus thermophilus , rna , nucleotide , crystal structure , chemistry , crystallography , nucleic acid structure , loop (graph theory) , stereochemistry , ribosomal rna , molecule , biochemistry , gene , escherichia coli , mathematics , organic chemistry , combinatorics
The synthetic RNA fragment 5′‐CUGGGCGG(GCGA)CCGCCUGG (nucleotides in parentheses indicate the loop region) corresponds to the natural sequence of domain E from nucleotides 79–97 of the Thermus flavus 5S rRNA including a hairpin loop. The RNA structure determined at 3.0 Å and refined to an R ‐value of 24.1% also represents the first X‐ray structure GNRA tetraloop. The loop is in distinctly different conformation from other GNRA tetraloops analyzed by NMR. The conformation of the two molecules in the asymmetric unit is influenced and stabilized by specific intermolecular contacts. The structural features presented here give evidence for the ability of RNA molecules to adapt to specific environments.