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Minimal tRNA Ser and tRNA Sec substrates for human seryl‐tRNA synthetase: contribution of tRNA domains to serylation and tertiary structure
Author(s) -
Heckl Martha,
Busch Kristina,
Gross Hans J
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00435-9
Subject(s) - transfer rna , t arm , amino acyl trna synthetases , rna , biology , biochemistry , protein tertiary structure , stereochemistry , chemistry , gene
The recognition process of tRNA Ser and tRNA Sec by human seryl‐tRNA synthetase (SerRS) was studied using T7 transcripts representing defined regions of human tRNA Ser or tRNA Sec and the influence of the tRNA elements on serylation and tertiary structure was elucidated. The anticodon arms of both tRNAs showed no contribution to serylation in contrast to the acceptor stems and the long extra arms. D and T arms were only involved in formation of the L‐shaped tRNA structure, not in the recognition process between tRNAs and SerRS. This is the first report of microhelices adapted from human tRNAs being aminoacylated by their homologous synthetase.