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Differential gene expression of a human α2,3‐sialyltransferase in leukaemic cell lines and leucocytes
Author(s) -
Gassen Uta,
Kelm Sørge,
Schauer Roland
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00407-4
Subject(s) - hl60 , sialyltransferase , ap 1 transcription factor , gene , cell culture , microbiology and biotechnology , gene expression , biology , genetics , glycoprotein
The gene expression of the human Galβ1,4(3)GlcNAc/Galβ1,3GalNAcα‐2,3‐sialyltransferase was investigated in the leukaemic cell lines HL60, K‐562, MOLT‐4, THP‐1 and in blood leucocytes. Five different transcripts were identified. In HL60 and THP‐1 cells the expression levels of two of these changed during differentiation. Two potential AP1 binding sites were detected in the promoter regions of the gene. THP‐1 cells contain proteins binding with higher affinities to these sequences in the sialyltransferase gene than to the AP1 consensus sequence, whereas nuclear extracts from HL60 cells have the opposite affinity.