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Biological dynamics and distribution of 3‐hydroxy fatty acids in the yeast Dipodascopsis uninucleata as investigated by immunofluorescence microscopy. Evidence for a putative regulatory role in the sexual reproductive cycle 1
Author(s) -
Kock J.L.F,
Venter P,
Linke D,
Schewe T,
Nigam S
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00406-2
Subject(s) - yeast , immunofluorescence , biology , dynamics (music) , reproductive cycle , distribution (mathematics) , microbiology and biotechnology , biochemistry , chemistry , reproduction , genetics , antibody , psychology , mathematical analysis , pedagogy , mathematics
Dipodascopsis uninucleata has been recently shown to produce 3‐hydroxy polyenoic fatty acids from several exogenous polyenoic fatty acids. In order to examine whether endogenous 3‐hydroxy fatty acids (3‐OH‐FA) may be implicated in the developmental biology of this yeast, we mapped by immunofluorescence microscopy their occurrence in fixed cells with or without cell walls using an antibody raised against 3 R ‐hydroxy‐5 Z ,8 Z ,11 Z ,14 Z ‐eicosatetraenoic acid (3 R ‐HETE), the biotransformation product from arachidonic acid (AA). This antibody turned out to cross‐react with other 3‐OH‐FA. 3‐OH‐FA were detected in situ in gametangia, asci, as well as between released ascospores, and proved to be associated with the sexual reproductive stage of the life cycle of the yeast. Acetylsalicylic acid (1 mM), which is known to suppress the formation of 3‐OH‐FA from exogenous polyenoic fatty acids, inhibited the occurrence of immunoreactive material as well as the sexual phase of the life cycle suggesting a prominent regulatory role of 3‐OH‐FA for the latter.