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Homologous p35 proteins of baculoviruses show distinctive anti‐apoptotic activities which correlate with the apoptosis‐inducing activity of each virus 1
Author(s) -
Morishima Nobuhiro,
Okano Kazuhiro,
Shibata Takehiko,
Maeda Susumu
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00389-5
Subject(s) - autographa californica , cleavage (geology) , apoptosis , in vitro , microbiology and biotechnology , chemistry , virus , biology , baculoviridae , virology , gene , recombinant dna , biochemistry , spodoptera , paleontology , fracture (geology)
The anti‐apoptotic activity of p35s from two baculoviruses, Autographa californica nucleopolyhedrovirus (AcNPV) and Bombyx mori NPV (BmNPV), was compared in mammalian cells. AcNPV p35 efficiently blocked apoptosis induced by caspase overexpression, but BmNPV p35 did so very poorly. Analysis of chimeric p35s and in vitro cleavage of wild type p35s suggest that the cleavage efficiency of p35 correlates with the blocking activity. Single amino acid substitutions of BmNPV p35 with those observed in AcNPV p35, however, resulted in significant loss of its anti‐apoptotic activity. We speculate that sequences flanking the cleavage site have uniquely evolved during baculovirus evolution.