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Xanthine oxidoreductase is asymmetrically localised on the outer surface of human endothelial and epithelial cells in culture
Author(s) -
Rouquette Magali,
Page Susanna,
Bryant Richard,
Benboubetra Mustapha,
Stevens Cliff R,
Blake David R,
Whish William D,
Harrison Roger,
Tosh David
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00385-8
Subject(s) - cytoplasm , microbiology and biotechnology , immunofluorescence , cell , cell culture , biology , oxidoreductase , umbilical vein , endothelial stem cell , human umbilical vein endothelial cell , chemistry , enzyme , biochemistry , immunology , antibody , in vitro , genetics
Subcellular localisation of xanthine oxidoreductase (XOR) was determined by indirect immunofluorescence using confocal microscopy in human endothelial and epithelial cell lines and in primary cultures of human umbilical vein endothelial cells. XOR was diffusely distributed throughout the cytoplasm but with higher intensity in the perinuclear region. In non‐permeabilised cells, XOR was clearly seen to be asymmetrically located on the outer surfaces, showing, in many cases, a higher intensity on those faces apposed by closely neighbouring cells. Such specific distribution suggests a functional role for the enzyme in cell‐cell interactions, possibly involving signalling via reactive oxygen species