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Carbohydrate induced modulation of cell membrane VII. Binding of exogenous lectin increases osmofragility of erythrocytes
Author(s) -
Pande Abhay H.,
Hajela Namita,
Hajela Krishnan
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00384-6
Subject(s) - agglutination (biology) , lectin , concanavalin a , hemolysis , chemistry , biochemistry , glycoprotein , receptor , tonicity , cell surface receptor , membrane , biophysics , biology , antibody , immunology , in vitro
Due to their multivalent binding character, lectins when added exogenously will cross‐link membrane surface receptors leading to lateral molecular reorganizations in the plane of the bilayer. This study reports for the first time that agglutination of rabbit erythrocytes by lentil lectin and concanavalin A increases their osmofragility. Increase in osmofragility was detected by measuring the hemolysis of erythrocytes in hypotonic as well as in isotonic solutions. It was also found that agglutination per se does not increase osmofragility but the binding of legume lectin is essential since human Rh + cells agglutinated by a monoclonal antibody do not exhibit hemolysis.