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Inhibition and enhancement of odorant‐induced cAMP accumulation in rat olfactory cilia by antibodies directed against G αs/olf ‐ and G αi ‐protein subunits
Author(s) -
Sinnarajah Srikumar,
Ezeh Patrick I,
Pathirana Suram,
Moss Anthony G,
Morrison Edward E,
Vodyanoy Vitaly
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00368-8
Subject(s) - adenylyl cyclase , cilium , immunoelectron microscopy , chemistry , gs alpha subunit , microbiology and biotechnology , protein subunit , antibody , olfactory system , g protein , blot , effector , biochemistry , enzyme , receptor , biology , neuroscience , immunology , gene
The odorant‐induced accumulation of cAMP can be inhibited by antibodies directed against G αs/olf . In contrast, antibodies raised against G αi ‐subunits caused a strong enhancement of the odorant‐induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both G αS/olf ‐ and G αi ‐subunits in rat cilia preparations. The existence of both stimulatory and inhibitory odorant‐induced regulation of adenylyl cyclase activity in olfactory cilia may indicate that an initial integration of different odorant stimuli begins at the level of primary reactions in the same effector enzyme.