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Direct formation of mixed micelles in the solubilization of phospholipid liposomes by Triton X‐100
Author(s) -
López Olga,
de la Maza Alfonso,
Coderch Luisa,
López-Iglesias Carmen,
Wehrli Ernst,
Parra Jose Luis
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00363-9
Subject(s) - micelle , vesicle , pulmonary surfactant , liposome , chemistry , bilayer , dynamic light scattering , phospholipid , phosphatidylcholine , octyl glucoside , chemical engineering , chromatography , solubilization , biophysics , membrane , organic chemistry , aqueous solution , biochemistry , nanoparticle , engineering , biology
The vesicle to micelle transition which results in the interaction of the Triton X‐100 surfactant with phosphatidylcholine vesicles was studied by means of dynamic light scattering (at different reading angles) and by freeze‐fracture electron microscopy techniques. Vesicle solubilization was produced by the direct formation of mixed micelles without the formation of complex intermediate aggregates. Thus, vesicle to micelle transformation was mainly governed by the progressive formation of mixed micelles within the bilayer. A subsequent separation of these micelles from the liposome surface (vesicle perforation by the formation of surfactant‐stabilized holes on the vesicle surface) led to a complete solubilization of liposomes.