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A cosmid and cDNA fine physical map of a human chromosome 13q14 region frequently lost in B‐cell chronic lymphocytic leukemia and identification of a new putative tumor suppressor gene, Leu5
Author(s) -
Kapanadze Bagrat,
Kashuba Vladimir,
Baranova Anna,
Rasool Omid,
van Everdink Wim,
Liu Yie,
Syomov Alexander,
Corcoran Martin,
Poltaraus Andrey,
Brodyansky Vadim,
Syomova Natalia,
Kazakov Alexey,
Ibbotson Rachel,
van den Berg Anke,
Gizatullin Rinat,
Fedorova Ludmila,
Sulimova Galina,
Zelenin Alexander,
Deaven Larry,
Lehrach Hans,
Grander Dan,
Buys Charles,
Oscier David,
Zabarovsky Eugene R.,
Einhorn Stephan,
Yankovsky Nick
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00357-3
Subject(s) - cosmid , contig , biology , genetics , homology (biology) , gene , locus (genetics) , microbiology and biotechnology , complementary dna , gene mapping , clone (java method) , chromosome , genome
B‐cell chronic lymphocytic leukemia (B‐CLL) is a human hematological neoplastic disease often associated with the loss of a chromosome 13 region between RB1 gene and locus D13S25. A new tumor suppressor gene (TSG) may be located in the region. A cosmid contig has been constructed between the loci D13S1168 (WI9598) and D13S25 (H2‐42), which corresponds to the minimal region shared by B‐CLL associated deletions. The contig includes more than 200 LANL and ICRF cosmid clones covering 620 kb. Three cDNAs likely corresponding to three different genes have been found in the minimally deleted region, sequenced and mapped against the contigged cosmids. cDNA clone 10k4 as well as a chimeric clone 13g3, codes for a zinc‐finger domain of the RING type and shares homology to some known genes involved in tumorigenesis (RET finger protein, BRCA1) and embryogenesis (MID1). We have termed the gene corresponding to 10k4/13g3 clones LEU5. This is the first gene with homology to known TSGs which has been found in the region of B‐CLL rearrangements.