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Increased cellular resistance to oxidative stress by expression of cyanobacterium catalase‐peroxidase in animal cells
Author(s) -
Ishikawa Takahiro,
Ohta Yuriko,
Takeda Toru,
Shigeoka Shigeru,
Nishikimi Morimitsu
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00347-0
Subject(s) - catalase , peroxidase , oxidative stress , peroxiredoxin , cytosol , enzyme , biochemistry , superoxide dismutase , biology , paraquat , microbiology and biotechnology , subcellular localization , chemistry , gene
To exploit prokaryotic antioxidant enzymes for protection of animal cells from oxidative damage, we expressed catalase‐peroxidase of cyanobacterium Synechococcus PCC 7942 in 104C1 cells. The gene for this enzyme was inserted into the mammalian expression vector pRc/CMV. The stable transfectants obtained had higher specific activities of catalase and as a result became more resistant to H 2 O 2 or paraquat than the parental cells. Subcellular fractionation and immunoblot analysis revealed that the expressed catalase‐peroxidase was confined to the cytosol; this localization may be the basis for the effective protection of the transfectants from the oxidative cell damage.