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HPLC determination of photosynthetic pigments during greening of etiolated barley leaves
Author(s) -
Nakamura Akimasa,
Watanabe Tadashi
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00344-5
Subject(s) - greening , p700 , photosynthesis , etiolation , pigment , chlorophyll , chlorophyll a , photosystem i , photosynthetic pigment , chloroplast , botany , chemistry , photosystem ii , photosystem , biology , biochemistry , ecology , organic chemistry , gene , enzyme
The temporal evolution of pigment composition during greening of etiolated barley leaves was investigated by reversed‐phase HPLC with particular attention to chlorophyll (Chl) a ′ (C13 2 epimer of Chl a ), which had been detected by ourselves in photosystem (PS) I particles. At early stages of greening, the Chl a ′/Chl a molar ratio rapidly increased to a level more than twice that in mature leaves, then gradually leveled off, accompanied by a growth of the Chl b /Chl a ratio, to the mature level. After 3 h of illumination, the temporal evolution of the Chl a ′/Chl a molar ratio nearly paralleled that of the P700/Chl a ratio with a stoichiometry Chl a ′/P700≅2: this strongly suggests that Chl a ′ is biosynthesized as a constituent of the PS I reaction center complex.