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The skin‐type antifreeze protein gene intron of the winter flounder is a ubiquitous enhancer lacking a functional C/EBPα binding motif
Author(s) -
Miao Ming,
Chan Shing-Leng,
Hew Choy L,
Gong Zhiyuan
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00327-5
Subject(s) - enhancer , intron , antifreeze protein , biology , gene , microbiology and biotechnology , ccaat enhancer binding proteins , gene expression , genetics , nuclear protein , transcription factor
The winter flounder antifreeze protein (AFP) intron contains a liver‐specific enhancer (Element B) which was shown earlier to bind CCAAT/enhancer binding protein (C/EBP)α. In contrast, as demonstrated in the present studies, the intron of the skin‐type AFP gene acted as a ubiquitous enhancer and contained a TA insertion at similar region to Element B (Element S) which destroyed its interaction with C/EBPα. Furthermore, a TA insertion of Element B by site‐directed mutagenesis decreased its liver enhancer activity. The presence or absence of C/EBPα binding motifs in Element B and Element S, respectively, may provide a mechanism for their differential expression.