Premium
Two distinct states of the thylakoid bf complex
Author(s) -
Heimann Sebastian,
Klughammer Christof,
Schreiber Ulrich
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00326-3
Subject(s) - thylakoid , chemistry , biophysics , biology , biochemistry , chloroplast , gene
Under normal physiological conditions the state of the cyt bf complex is characterized by rapid reoxidation kinetics of cyt b ‐563 following flash‐illumination. It is known that these kinetics are dramatically slowed down under oxidizing conditions. Here we show that this slow‐down of cyt b ‐563 oxidation is the consequence of a relatively slow (half‐time of several minutes) transformation of the cyt bf complex into a distinctly different state (termed state‐s). Reversal to the normal state requires strong reductive treatment or light‐induced electron transport. The results are in line with a recent model of functional cyt bf dimers [Cramer et al., Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 (1996), 477–508], if it is assumed that state‐s reflects the monomeric state of the bf complex.