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Oct‐1 promoter region contains octamer sites and TAAT motifs recognized by Oct proteins
Author(s) -
Pankratova E.V,
Polanovasky O.L
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00316-0
Subject(s) - pou domain , homeobox , transcription factor , histone octamer , promoter , gene , biology , microbiology and biotechnology , promoter activity , reporter gene , genetics , gene expression , nucleosome , histone
The 5′‐upstream region (1.3 kb) of the gene encoding the POU domain transcription factor Oct‐1 was cloned and sequenced. CAT reporter gene analysis of this region has detected a functionally active promoter. This region contains 24 TAAT‐core sites, arranged in five clusters (four to six sites in one cluster); two octamer sites (ATGCAAAT) are located in the first and second clusters; in the second one the CCAAT‐box adjacent to the octamer overlaps with the TAAT‐core site. As shown by gel retardation assay, Oct‐1, Oct‐2, and some unknown proteins from myeloma cell line NS/0 interact with the TAAT‐core sites of these clusters. The results suggest autoregulation of Oct‐1 gene expression that may also be controlled by other POU proteins, homeodomain proteins and CCAAT trans ‐action factors.