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Zeatin is indispensable for the G 2 ‐M transition in tobacco BY‐2 cells
Author(s) -
Laureys Françoise,
Dewitte Walter,
Witters Erwin,
Van Montagu Marc,
Inzé Dirk,
Van Onckelen Harry
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00297-x
Subject(s) - zeatin , mevalonic acid , cytokinin , auxin , lovastatin , mitosis , nicotiana tabacum , biochemistry , transition (genetics) , biosynthesis , cell division , biology , chemistry , microbiology and biotechnology , cell , enzyme , gene , cholesterol
LC‐MS/MS quantification of endogenous cytokinins proved that lovastatin affects cytokinin biosynthesis by inhibiting HMG‐CoA reductase. Out of eight different aminopurines and a synthetic auxin tested for their ability to override lovastatin inhibition of mitosis, only zeatin was active. Our data point to a key role for a well‐defined cytokinin (here, zeatin) in the G 2 ‐M transition of tobacco BY‐2 cells.