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Orientation of antigen binding sites in dimeric and trimeric single chain Fv antibody fragments
Author(s) -
Lawrence Lynne J.,
Kortt Alexander A.,
Iliades Peter,
Tulloch Peter A.,
Hudson Peter J.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00292-0
Subject(s) - chemistry , linker , immunoglobulin fab fragments , stereochemistry , molecule , immunoglobulin light chain , crystallography , antibody , microbiology and biotechnology , complementarity determining region , peptide sequence , biochemistry , biology , organic chemistry , computer science , immunology , gene , operating system
Electron microscopy of dimeric and trimeric single chain antibody Fv fragments (scFvs) complexed with anti‐idiotype Fab fragments was used to reveal the orientation of antigen binding sites. This is the first structural analysis that discloses the multivalent binding orientation of scFv trimers (triabodies). Three different scFv molecules were used for the imaging analysis; NC10 scFv‐5 and scFv‐0, with five‐ and zero‐residue linkers respectively between the V H and V L domains, were complexed with 3‐2G12 anti‐idiotype Fab fragments and 11‐1G10 scFv‐0 was complexed with NC41 anti‐idiotype Fab fragments. The scFv‐5 molecules formed bivalent dimers (diabodies) and the zero‐linker scFv‐0 molecules formed trivalent trimers (triabodies). The images of the NC10 diabody‐Fab complex appear as boomerangs, not as a linear molecule, with a variable angle between the two Fab arms and the triabody‐Fab complexes appear as tripods.