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Hexokinase PII has a double cytosolic‐nuclear localisation in Saccharomyces cerevisiae
Author(s) -
Randez-Gil Francisca,
Herrero Pilar,
Sanz Pascual,
Prieto José Antonio,
Moreno Fernando
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00289-0
Subject(s) - hexokinase , saccharomyces cerevisiae , cytoplasm , cytosol , biochemistry , mutant , yeast , chemistry , gene product , green fluorescent protein , microbiology and biotechnology , biology , enzyme , gene , gene expression , glycolysis
We describe here that the HXK2 gene product, isoenzyme PII of hexokinase, is located in both the nucleus and the cytoplasm of Saccharomyces cerevisiae cells. This conclusion is supported by assays of hexokinase‐specific activity in isolated nuclei from wild‐type and hxk1/hxk2 double mutant strains, by immunoblot experiments using anti‐Hxk2 antibodies and by observation of the fluorescence distribution of a Hxk2‐GFP fusion protein in cells transformed with the HXK2::gfp gene.