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Regulation of the glial glutamate transporter GLT‐1 by glutamate and δ‐opioid receptor stimulation
Author(s) -
Thorlin Thorleif,
Roginski Raymond S,
Choudhury Kanakendu,
Nilsson Michael,
Rönnbäck Lars,
Hansson Elisabeth,
Eriksson Peter S
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00288-9
Subject(s) - glutamate receptor , glutamate aspartate transporter , metabotropic glutamate receptor 6 , chemistry , metabotropic glutamate receptor , metabotropic glutamate receptor 7 , agonist , astrocyte , stimulation , metabotropic glutamate receptor 2 , receptor , microbiology and biotechnology , biochemistry , biology , endocrinology , central nervous system
The excitatory effect of presynaptically released glutamate is tightly regulated and terminated by high affinity sodium‐dependent glutamate transporters. The regulation of the glial glutamate transporter GLT‐1 is potentially important in synaptic modulation. Using astroglial cultures prepared from the rat cerebral cortex, we found that the δ‐opioid receptor agonist [ d ‐pen 2 , d ‐pen 5 ]‐enkephalin decreases and glutamate increases the expression of the GLT‐1 transporter mRNA. Corresponding changes in the uptake kinetics were found after incubation for 48 h with the respective agonists when glial glutamate uptake was measured in primary astroglial cultures. The data suggest that long‐term receptor activation induces alterations in glial glutamate uptake properties.