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hUBC9 associates with MEKK1 and type I TNF‐α receptor and stimulates NFκB activity
Author(s) -
Saltzman Alan,
Searfoss George,
Marcireau Christophe,
Stone Maureen,
Ressner Rose,
Munro Robin,
Franks Carol,
D'Alonzo Jill,
Tocque Bruno,
Jaye Michael,
Ivashchenko Yuri
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00287-7
Subject(s) - nf κb , chemistry , tumor necrosis factor alpha , hela , receptor , ubiquitin , secretion , reporter gene , signal transduction , microbiology and biotechnology , biology , biochemistry , in vitro , endocrinology , gene , gene expression
hUBC9, an E2 ubiquitin conjugating enzyme, was identified by yeast two‐hybrid screening and coprecipitation studies to interact with MEKK1 and the type I TNF‐α receptor, respectively. Because both of these proteins regulate NFκB activity, the role of hUBC9 in modulating NFκB activity was investigated. Overexpression of hUBC9 in HeLa cells stimulated the activity of NFκB as determined by NFκB reporter and IL‐6 secretion assays. hUBC9 also synergized with MEKK1 to activate NFκB reporter activity. Thus, hUBC9 modulates NFκB activity which, at least in part, can be attributed to its interaction with MEKK1 and the type I TNF‐α receptor.