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Further assignment of resonances in 1 H NMR spectra of cerebrospinal fluid (CSF)
Author(s) -
Lutz Norbert W,
Maillet Serge,
Nicoli François,
Viout Patrick,
Cozzone Patrick J
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00262-2
Subject(s) - spectral line , chemistry , cerebrospinal fluid , nuclear magnetic resonance , nmr spectra database , nuclear magnetic resonance spectroscopy , spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , stereochemistry , chromatography , physics , medicine , pathology , quantum mechanics , astronomy
A number of previously unidentified 1 H NMR signals detected in CSF spectra of patients with various neurological and metabolic diseases are assigned to metabolites, drugs and drug excipients. Two‐dimensional 1 H NMR spectroscopy (COSY and J‐resolved) is employed to resolve resonances which are hidden by superimposed peaks in one‐dimensional spectra. Assignments obtained by making use of 2‐D techniques, and of a 1‐D 1 H NMR data base created for ca. 150 authentic compounds, enable us to clarify the nature of complex signal patterns found in crowded spectral regions of CSF such as the aliphatic methyl region at ca. 1.0 ppm.