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Glucagon‐like peptide‐1 receptor expression in Xenopus oocytes stimulates inositol trisphosphate‐dependent intracellular Ca 2+ mobilization
Author(s) -
Gromada Jesper,
Anker Charlotte,
Bokvist Krister,
Knudsen Lotte B,
Wahl Philip
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00254-3
Subject(s) - inositol , xenopus , forskolin , bapta , signal transduction , receptor , inositol trisphosphate receptor , intracellular , activator (genetics) , endocrinology , medicine , chemistry , glucagon , inositol phosphate , biology , microbiology and biotechnology , biochemistry , hormone , gene
The signal transduction pathway of the cloned human glucagon‐like peptide‐1 (GLP‐1) receptor was studied in voltage‐clamped Xenopus oocytes. Binding of GLP‐1(7–36)amide was associated with cAMP production, increased [Ca 2+ ] i and activation of Ca 2+ ‐dependent Cl − current. The effect of GLP‐1(7–36)amide reflects intracellular Ca 2+ mobilization and was suppressed by injection of the Ca 2+ chelator BAPTA and the inositol trisphosphate receptor antagonist heparin. The responses were not mimicked by the adenylate cyclase activator forskolin and unaffected by the protein kinase A (PKA) inhibitor Rp‐cAMPS. We conclude that GLP‐1 receptor expression in Xenopus oocytes evokes inositol trisphosphate‐dependent intracellular Ca 2+ mobilization independent of the cAMP/PKA signaling pathway.