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Cloning, sequencing and expression of the genes encoding the sodium translocating N 5 ‐methyltetrahydromethanopterin:coenzyme M methyltransferase of the methylotrophic archaeon Methanosarcina mazei Gö1
Author(s) -
Lienard Tanja,
Gottschalk Gerhard
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00229-4
Subject(s) - corrinoid , methyltransferase , methanosarcina , biochemistry , archaea , transmethylation , methanosarcina barkeri , chemistry , biology , gene , methylation , methanogenesis , genetics , bacteria
The N 5 ‐methyltetrahydromethanopterin:coenzyme M methyltransferase of Methanosarcina mazei Gö1 is a membrane‐associated, corrinoid‐containing protein that uses a transmethylation reaction to drive an energy‐conserving sodium ion pump. The eight open reading frames encoding the eight different subunits of the methyltransferase were identified and sequenced. All of these subunits are shown to be heterologously expressed in minicells of the Escherichia coli mutant DK6. Sequence comparisons with the methyltransferases of thermophilic and hypothermophilic methanogenic archaea are presented. The participation of the gene product of mtr D in sodium ion translocation as well as a consensus sequence of a corrinoid binding motif in MtrA are discussed.