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A somatostatin receptor 1 selective ligand inhibits Ca 2+ currents in rat insulinoma 1046‐38 cells
Author(s) -
Roosterman Dirk,
Glassmeier Günter,
Baumeister Hans,
Scherübl Hans,
Meyerhof Wolfgang
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00221-x
Subject(s) - somatostatin , insulinoma , somatostatin receptor , receptor , endocrinology , medicine , adenylyl cyclase , chemistry , biology , microbiology and biotechnology , insulin
Rat insulinoma 1046‐38 cells represent a model system to study β‐cell function. The mRNAs for sst1 and sst2, two of the five somatostatin receptors, were detected by reverse transcription polymerase chain reaction amplification in these cells. Displacement binding analysis suggested that sst1 represents the major somatostatin receptor subtype. The sst1 selective compound CH‐275 did not inhibit adenylyl cyclases while compounds that activated sst2 did. In contrast, CH‐275 caused a marked inhibition of voltage‐operated Ca 2+ channels while the sst2 specific analog octreotide elicited a less pronounced effect suggesting that in rat insulinoma 1046‐38 cells sst1 preferably mediates the inhibition of Ca 2+ channels.