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Folding and binding activity of the 3′UTRs of Paracentrotus lividus bep messengers
Author(s) -
Montana Giovanna,
Sbisà Elisabetta,
Romancino Daniele P,
Bonura Angela,
Di Carlo Marta
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00215-4
Subject(s) - paracentrotus lividus , microinjection , messenger rna , microbiology and biotechnology , biology , untranslated region , folding (dsp implementation) , rna , translation (biology) , gene , genetics , sea urchin , engineering , electrical engineering
Bep mRNAs are localized at the animal pole of P. lividus eggs. In the present communication the secondary structures of the 3′UTRs of the bep1 , bep3 and bep4 mRNAs are reported. The minimal lenghts of these regions required to bind the 54‐kDa protein, previously shown to be involved in localization and anchoring of these RNAs, is estimated. Microinjection of the bep3 3′UTR into egg shows that this RNA fragment is also able to become localized to one of the egg poles, as happens for the entire bep3 RNA.