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Identification of an ABC transporter gene that exhibits mRNA level overexpression in fluoroquinolone‐resistant Mycobacterium smegmatis
Author(s) -
Banerjee Sanjiban K,
Misra Parimal,
Bhatt Kamlesh,
Mande Shekhar C,
Chakraborti Pradip K
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00214-2
Subject(s) - mycobacterium smegmatis , atp binding cassette transporter , gene , transporter , biology , amino acid transporter , homology (biology) , microbiology and biotechnology , genetics , biochemistry , mycobacterium tuberculosis , medicine , tuberculosis , pathology
We describe here the PCR amplification of a DNA fragment ( mtp1 ) from Mycobacterium smegmatis using primers derived from consensus sequences of the ABC family of transporters. The fragment encodes amino acid sequences that exhibited significant homology with different ABC transporters. Amino acid sequence alignment of the full length gene with other transporters identified the ABC protein as the B‐subunit of the phosphate specific transporter. Strikingly, a M. smegmatis colony which exhibited a high level of ciprofloxacin resistance showed mRNA level overexpression of mtp1 . Thus this is the first report in any prokaryote indicating differential expression of an ABC transporter in a fluoroquinolone resistant colony.