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Altered expression of α‐actin, smooth muscle myosin heavy chain‐1 and calponin in cultured smooth muscle cells by oxidized low density lipoproteins
Author(s) -
Damiani Elisabetta,
Sugiyama Tomoyasu,
Shimamura Kyoko,
Greci Lucedio,
Matsuda Yuzuru
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00212-9
Subject(s) - calponin , actin , myosin , smooth muscle tissue , myosin light chain kinase , microbiology and biotechnology , vascular smooth muscle , chemistry , cell , smooth muscle , cell culture , myocyte , biochemistry , biology , endocrinology , genetics
The expression of the contractile proteins, α‐actin, smooth muscle myosin heavy chain‐1 (SM1) and calponin present in smooth muscle cells (SMC) in the presence of oxidized low density lipoproteins (oxLDL) was investigated in two different cell cultures: the mouse smooth muscle cell line SVSC and rat smooth muscle cells (RSMC). Exposure of the cells to 187 μg protein/ml oxLDL for 24 h reduced the expression of all three contractile proteins in both cell cultures when compared to cells incubated in the presence of native LDL. This investigation of the response of SMC contractile proteins to oxLDL may provide further insights into the mechanisms by which oxidatively modified LDL is atherogenic and suggests that oxLDL may contribute to the regulation of the expression of the genes responsible for the synthesis of smooth muscle cell contractile proteins.