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Transformation system for prototrophic industrial yeasts using the AUR1 gene as a dominant selection marker
Author(s) -
Hashida-Okado Takashi,
Ogawa Atsuko,
Kato Ikunoshin,
Takesako Kazutoh
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00211-7
Subject(s) - auxotrophy , transformation (genetics) , biology , kluyveromyces lactis , plasmid , gene , genetics , yeast , saccharomyces cerevisiae , ploidy , kluyveromyces , candida glabrata , selection (genetic algorithm) , candida albicans , mutant , artificial intelligence , computer science
We show a new transformation system for prototrophic yeast strains including those of Saccharomyces cerevisiae , Kluyveromyces lactis , K. marxianus , and Candida glabrata . This system is composed of an antibiotic, aureobasidin A (AbA), and its resistance gene AUR1 ‐C as a selection marker. Southern analysis of genomic DNAs of the transformants indicated that the copy number of the plasmid increased from one to more than four, depending on the concentration of AbA used for selection of the transformants. The AUR1 ‐C gene was also effective as a selection marker for gene disruption, and was able to disrupt both copies of the gene on homologous chromosomes of diploid cells by a single round of transformation. This system has a broad application in the transformation and gene disruption of prototrophic strains of a variety of yeast species.