Premium
Large quantity production with extreme convenience of human SDF‐1α and SDF‐1β by a Sendai virus vector
Author(s) -
Moriya Chikaya,
Shioda Tatsuo,
Tashiro Kei,
Nagasawa Takashi,
Ikegawa Masaya,
Ohnishi Yukano,
Kato Atsushi,
Hu Huiling,
Xin Xiaomi,
Hasan Mohammad K,
Maekawa Midori,
Takebe Yutaka,
Sakai Yuko,
Honjo Tasuku,
Nagai Yoshiyuki
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00207-5
Subject(s) - sendai virus , production (economics) , vector (molecular biology) , virus , virology , biology , biochemistry , recombinant dna , economics , macroeconomics , gene
We describe a robust expression of human stromal cell‐derived factor‐1α (SDF‐1α) and SDF‐1β, the members of CXC‐chemokine family, with a novel vector system based upon Sendai virus, a non‐segmented negative strand RNA virus. Recombinant SDF‐1α and SDF‐1β were detected as a major protein species in culture supernatants, reached as high as 10 μg/ml. This remarkable enrichment of the products allowed us to use even the crude supernatants as the source for biological and antiviral assays without further concentration nor purification and will thus greatly facilitate to screen their genetically engineered derivatives.