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Molecular cloning and characterization of two novel transport proteins from rat kidney
Author(s) -
Schömig Edgar,
Spitzenberger Folker,
Engelhardt Martin,
Martel Fátima,
Örding Nicola,
Gründemann Dirk
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00203-8
Subject(s) - major facilitator superfamily , solute carrier family , transporter , homology (biology) , cloning (programming) , sequence homology , organic anion transporter 1 , chemistry , molecular cloning , microbiology and biotechnology , kidney , biology , polymerase chain reaction , biochemistry , peptide sequence , gene , genetics , computer science , programming language
The recent cloning of renal transport systems for organic anions and cations (OAT1, OCT1, and OCT2) opened the possibility to search, via polymerase chain reaction (PCR) homology screening, for novel transport proteins. Two integral membrane proteins, UST1 and UST2, were cloned from rat kidney. RT‐PCR revealed that UST1 is confined to the kidney whereas UST2 mRNA was detected in all tested tissues. Sequence analyses suggest that UST1 and UST2, together with four related transporters, comprise, within the major facilitator superfamily, a so far unrecognized transporter family, termed amphiphilic solute facilitator (ASF) family. Characteristic signatures for the ASF family were identified.