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Neurotrophin‐7: a novel member of the neurotrophin family from the zebrafish
Author(s) -
Nilsson Ann-Sofie,
Fainzilber Michael,
Falck Pierre,
Ibáñez Carlos F
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00192-6
Subject(s) - neurotrophin , neurotrophin 3 , tropomyosin receptor kinase a , biology , tropomyosin receptor kinase c , trk receptor , low affinity nerve growth factor receptor , nerve growth factor , zebrafish , peptide sequence , microbiology and biotechnology , tyrosine , tropomyosin receptor kinase b , receptor , neurotrophic factors , genetics , brain derived neurotrophic factor , biochemistry , platelet derived growth factor receptor , gene , growth factor
A novel member of the neurotrophin family, zebrafish neurotrophin‐7 (zNT‐7), was isolated from the zebrafish Danio rerio . The amino acid sequence of zNT‐7 is more closely related to that of fish nerve growth factor (NGF) and neurotrophin‐6 (NT‐6) than to that of any other neurotrophin. zNT‐7 is, however, equally related to fish NGF and NT‐6 (65% and 63% amino acid sequence identity, respectively) indicating that it represents a distinct neurotrophin sequence. zNT‐7 contains a 15 amino acid residue insertion in a β‐turn region in the middle of the mature protein. Recombinant zNT‐7 was able to bind to the human p75 neurotrophin receptor and to induce tyrosine phosphorylation of the rat TrkA receptor tyrosine kinase, albeit less efficiently than rat NGF. zNT‐7 did not interact with rat TrkB or TrkC, indicating a similar receptor specificity as NGF. We propose that a diversification of the NGF subfamily in the neurotrophin evolutionary tree occurred during the evolution of teleost fishes which resulted in the appearance of several additional members, such as zNT‐7 and NT‐6, structurally and functionally related to NGF.