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A new family of orphan G protein‐coupled receptors predominantly expressed in the brain 1
Author(s) -
Valdenaire Olivier,
Giller Thomas,
Breu Volker,
Ardati Ali,
Schweizer Anja,
Richards J.Grayson
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00170-7
Subject(s) - complementary dna , receptor , microbiology and biotechnology , endothelin receptor , biology , peptide sequence , g protein coupled receptor , biochemistry , gene
The cloning of a cDNA encoding a G protein‐coupled receptor homologous to the endothelin type B receptor, but unable to bind endothelin, was recently reported and termed ET B R‐LP. We report here the isolation of a human cDNA encoding a receptor that is highly related to ET B R‐LP and which was therefore termed ET B R‐LP‐2. Comparison of the two amino acid sequences revealed 68% overall homology and 48% identity. As is the case for ET B R‐LP, the new receptor is strongly expressed in the human central nervous system (e.g. in cerebellar Bergmann glia, cerebral cortex, internal capsule fibers). Membranes of HEK‐293 cells stably expressing ET B R‐LP‐2 did not bind endothelin‐1, endothelin‐2, endothelin‐3, bombesin, cholecystokinin‐8 or gastrin‐releasing peptide.