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A novel subtype of G‐protein‐coupled receptor kinase, GRK7, in teleost cone photoreceptors
Author(s) -
Hisatomi Osamu,
Matsuda Shinji,
Satoh Takunori,
Kotaka Shuichi,
Imanishi Yoshikazu,
Tokunaga Fumio
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00162-8
Subject(s) - biology , rhodopsin , opsin , g protein coupled receptor kinase , kinase , complementary dna , peptide sequence , oryzias , microbiology and biotechnology , g protein coupled receptor , retinal , genetics , biochemistry , signal transduction , gene
Two kinds of retinal cDNA fragments (OlGRK‐R and ‐C) encoding the putative G‐protein‐coupled receptor kinases (GRKs) were isolated from medaka, Oryzias latipes . OlGRK‐R appears to be closely related to the rhodopsin kinase (RK) found in the outer segments of mammalian photoreceptors, but the deduced amino acid sequence of OlGRK‐C shows less than 50% identity to those of GRKs known to date, suggesting that OlGRK‐C is a novel GRK subtype (GRK7). The mRNA of OlGRK‐R is detectable in rods, and that of OlGRK‐C is found in all four types of cone photoreceptor. The C‐terminal of OlGRK‐R has a consensus sequence for farnesylation, whereas, surprisingly, OlGRK‐C has a consensus sequence for geranylgeranylation. Our result are consistent with the concept that lower vertebrates have rod‐ and cone‐specific opsin kinases.