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Phospholamban domain I/cytochrome b 5 transmembrane sequence chimeras do not inhibit SERCA2a
Author(s) -
Kimura Yoshihiro,
Asahi Michio,
Kurzydlowski Kazimierz,
Tada Michihiko,
MacLennan David H
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00151-3
Subject(s) - phospholamban , transmembrane domain , transmembrane protein , inhibitory postsynaptic potential , endoplasmic reticulum , biology , chemistry , microbiology and biotechnology , biochemistry , membrane , receptor , neuroscience
A series of chimeras between the transmembrane domains of phospholamban (PLN) and cytochrome b 5 were coexpressed with the Ca 2+ ‐ATPase of cardiac sarcoplasmic reticulum (SERCA2a). The chimeric molecules were not inhibitory, in line with our view that inhibitory PLN/SERCA2a interactions occur in transmembrane sequences, while cytoplasmic interactions regulate the inhibitory interactions in a four‐base circuit.