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Vitronectin‐dependent invasion of epithelial cells by Neisseria gonorrhoeae involves α v integrin receptors
Author(s) -
Dehio Michaela,
Gómez-Duarte Oscar G,
Dehio Christoph,
Meyer Thomas F
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00144-6
Subject(s) - internalization , vitronectin , integrin , protein kinase c , microbiology and biotechnology , receptor , neisseria gonorrhoeae , neisseria , chemistry , kinase , biology , biochemistry , bacteria , genetics
Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa 50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN‐binding integrin receptors and protein kinase C (PKC) in VN‐triggered bacterial uptake. Blocking integrin function by RGDS peptides or by antibodies specific to α v β 5 or α v β 3 resulted in an abrogation of VN‐triggered bacterial internalization. Moreover, inhibitors of PKC were found to block VN‐triggered uptake. The essential role of α v integrins and the presumable involvement of PKC in VN‐triggered gonococcal uptake are discussed.