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Presence of leptin receptors in rat small intestine and leptin effect on sugar absorption
Author(s) -
Lostao M.Pilar,
Urdaneta Elena,
Martı́nez-Ansó Eduardo,
Barber Ana,
Martı́nez J.Alfredo
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00110-0
Subject(s) - leptin , medicine , endocrinology , chemistry , receptor , small intestine , leptin receptor , lamina propria , phlorizin , ileum , biology , biochemistry , glucose transporter , insulin , epithelium , obesity , genetics
Leptin is involved in food intake and thermogenesis regulation. Since leptin receptor expression has been found in several tissues including small intestine, a possible role of leptin in sugar absorption by the intestine was investigated. Leptin inhibited d ‐galactose uptake by rat small intestinal rings 33% after 5 min of incubation. The inhibition increased to 56% after 30 min. However, neither at 5 min nor at 30 min did leptin prevent intracellular galactose accumulation. This leptin effect was accompanied by a decrease of the active sugar transport apparent V max (20 vs. 4.8 μmol/g wet weight 5 min) and apparent K m (15.8 vs. 5.3 mM) without any change in the phlorizin‐resistant component. On the other hand, immunohistochemical experiments using anti‐leptin monoclonal antibodies recognized leptin receptors in the plasma membrane of immune cells located in the lamina propria. These results indicate for the first time that leptin has a rapid inhibitory effect on sugar absorption and demonstrate the presence of leptin receptors in the intestinal mucosa.