Premium
Interaction of peroxynitrite with carotenoids and tocopherols within low density lipoprotein
Author(s) -
Pannala Ananth Sekher,
Rice-Evans Catherine,
Sampson Julia,
Singh Surinder
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00108-2
Subject(s) - peroxynitrite , carotenoid , chemistry , low density lipoprotein , lipoprotein , food science , biochemistry , cholesterol , enzyme , superoxide
Peroxynitrite is a powerful oxidising and nitrating agent generated in vivo by the combination of nitric oxide and superoxide. Previous studies have shown that on exposure to peroxynitrite, low density lipoprotein (LDL) is modified resulting in both a time‐ and concentration‐dependent change to lipid and protein components. The present investigation highlights the reaction between carotenoids and tocopherols, present within the lipophilic phase of LDL, and peroxynitrite at varying concentrations. It was observed that the carotenoids were consumed by a significantly greater proportion than that of the tocopherols with lycopene (87.2±11%) being more reactive than β‐carotene (68.2±5.8%) when exposed to peroxynitrite (50 μM) for 1 min. Among the tocopherols, α‐tocopherol (54.9±20.2%) was more extensively depleted than γ‐tocopherol (14.7±1.09%) at peroxynitrite concentration of 500 μM. It was also observed that peroxynitrite, unlike copper ions, does not lead to significant peroxidation of LDL as determined by the formation of conjugated dienes and thiobarbituric acid‐reactive substances.